mouse anti arc (c-7)  (Santa Cruz Biotechnology)

Journal: Frontiers in Cell and Developmental Biology

Article Title: Placental trophoblast aging in advanced maternal age is related to increased oxidative damage and decreased YAP

doi: 10.3389/fcell.2025.1479960

Figure Lengend Snippet: AMA placentas showed a aging phenotype and were associated with decreased YAP and increased DNA oxidative damage. (A) SA-β-Gal staining of human term placenta sections (n = 10 patients per group, 3 random fields per patient). Scale bars, 100 μm. (B) IHC staining of p53, p21 and p16 in human term placentas (n = 10, 3 fields per patient). Scale bars, 50 μm. (C) Western blotting of p53, p21 and p16 protein expression in human term placentas (n = 6). (D) Western blotting of YAP protein expression in human term placentas (n = 6). (E) IHC staining of 8-OHdG in human term placentas (n = 10, 3 fields per patient). All data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01. Student’s t test. AU, arbitrary unit.

Article Snippet: 2,947, Cell Signaling, Danvers, United States), mouse mAb against p16 (1:50, Cod. sc-166760, Santa Cruz, Dallas, United States), mouse mAb against 8-OHdG (1:50, Cod. sc-393871, Santa Cruz, Dallas, United States) and PBS for negative control at 4°C overnight, followed by treatment with the secondary antibody (1: 200, Cod.

Techniques: Staining, Immunohistochemistry, Western Blot, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Placental trophoblast aging in advanced maternal age is related to increased oxidative damage and decreased YAP

doi: 10.3389/fcell.2025.1479960

Figure Lengend Snippet: YAP deficiency resulted in senescence and DNA oxidative damage in HTR8/SVneo cells, and compromised its invasiveness. (A) Western blotting of YAP expression in different HTR8/SVneo cells. sh-NC, negative control cells transfected with scramble shRNA; sh-YAP, cells transfected with shRNAs targeting YAP (n = 3). (B) Western blotting of YAP, p53, p21 and p16 expression in different HTR8/SVneo cells. Sh-YAP#1 was used in the following experiments (n = 3). (C) Representative SA-β-Gal staining in various HTR8/SVneo cells (n = 3). Scale bars, 100 μm. (D) Wound-healing assay of HTR8/SVneo cells (n = 3). Scale bars, 100 μm. (E) Transwell invasion assay of HTR8/SVneo cells (n = 3). Scale bars, 250 μm. (F) IF staining of YAP (red) and 8-OHdG (green) in HTR8/SVneo cells; nuclei were counterstained with DAPI (blue) (n = 3). Scale bar, 100 μm. All data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. Student's t test. AU, arbitrary unit. All data are representative of three independent experiments.

Article Snippet: 2,947, Cell Signaling, Danvers, United States), mouse mAb against p16 (1:50, Cod. sc-166760, Santa Cruz, Dallas, United States), mouse mAb against 8-OHdG (1:50, Cod. sc-393871, Santa Cruz, Dallas, United States) and PBS for negative control at 4°C overnight, followed by treatment with the secondary antibody (1: 200, Cod.

Techniques: Western Blot, Expressing, Negative Control, Transfection, shRNA, Staining, Wound Healing Assay, Transwell Invasion Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Placental trophoblast aging in advanced maternal age is related to increased oxidative damage and decreased YAP

doi: 10.3389/fcell.2025.1479960

Figure Lengend Snippet: Supplement of YAP ameliorated aging and DNA oxidative damage induced by H 2 O 2 in HTR8/SVneo cells. (A) Western blotting of YAP expression in different HTR8/SVneo cells (n = 3). OE-NC, negative control; OE-YAP, YAP overexpression. (B) Western blotting of YAP, p53, p21, and p16 expression in different HTR8/SVneo cells. OE-YAP#1 was used in the following experiments (n = 3). (C) Representative SA-β-Gal staining in various HTR8/SVneo cells (n = 3). Scale bars, 100 μm. OE-NC + H 2 O 2 , negative control cells treated with H 2 O 2 ; OE-YAP + H 2 O 2 , YAP overexpression cells treated with H 2 O 2 . (D) IF staining of YAP (red) and 8-OHdG (green) in HTR8/SVneo cells; nuclei were counterstained with DAPI (blue) (n = 3). Scale bar, 100 μm. All data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, nonsignificant. Student’s t-test for two groups, and one-way ANOVA for three or more groups. AU, arbitrary unit. All data are representative of three independent experiments.

Article Snippet: 2,947, Cell Signaling, Danvers, United States), mouse mAb against p16 (1:50, Cod. sc-166760, Santa Cruz, Dallas, United States), mouse mAb against 8-OHdG (1:50, Cod. sc-393871, Santa Cruz, Dallas, United States) and PBS for negative control at 4°C overnight, followed by treatment with the secondary antibody (1: 200, Cod.

Techniques: Western Blot, Expressing, Negative Control, Over Expression, Staining

Journal: iScience

Article Title: Growth-suppressor microRNAs mediate synaptic overgrowth and behavioral deficits in Fragile X mental retardation protein deficiency

doi: 10.1016/j.isci.2023.108676

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal Anti-Arc , Santa Cruz , sc-17839, Clone C-7.

Techniques: Virus, Generated, Plasmid Preparation, Lysis, TaqMan microRNA Assay, Bicinchoninic Acid Protein Assay, RNA Sequencing, CRISPR, Sequencing, Control, Recombinant, shRNA, Software